THE BIOLOGY OF HPV+ CANCER
Engineered
into a Blood Test.
Abstracts & Posters
Rapid Clearance of Circulating Tumor DNA Is Associated with Improved Survival in HPV Associated Oropharyngeal Squamous Cell Carcinoma
Birkenbeuel, J.; Lin, A.; Brown, K.; Miller, L.; Ozer, E.; Agrawal, A.; VanKoevering, K.; Seim, N.; Baliga, S.; Gogineni, E. In Proceedings of the American Head & Neck Society 2026.
Thematic Analysis of Patient Experiences with ctHPV DNA-Guided HPV-Positive Oropharyngeal Cancer
Fleming, G.; Roth, H.; Hanna, G.; Schoenfeld, J.; Minken, J.; Mukundan, D.; Windon, M.; Rettig, E. In Proceedings of the American Head & Neck Society 2026.
Transoral Resection and Circulating Tumor DNA Monitoring in HPV-Related Oropharyngeal Squamous Cell Carcinoma
Hintelmann, C.; Abend, A.; Imanguli, M.; Tang, D.; Casper, N.; Urego, L.; Caltabellotta, N.; Roden, D. In Proceedings of the American Head & Neck Society 2026.
TTMV Score at Molecular Recurrence Predicts Response to Salvage Therapy in HPV-Driven Cancers
Jabalee, J.; Kuperwasser, C.; Naber, S.; Berger, B.; Gupta, P. In Proceedings of the American Head & Neck Society 2026.
Characterizing Specificity and Sensitivity of Oral HPV DNA Testing among HPV-Positive Oropharynx Cancer Patients and Controls: The SPOT-HPV Study
Miller, J.; Sargent, B.; Hanna, G.; Schoenfeld, J.; Rettig, E. In Proceedings of the American Head & Neck Society 2026.
Lead Time Detection of Recurrent HPV Associated Oropharyngeal Squamous Cell Carcinoma Utilizing Circulating Tumor HPV DNA Testing
Sicard, R.; Yang, A.; Gomez, E.; Kraft, D.; Cooke, P.; Berger, M.; Teng, M.; Khan, M.; Genden, E.; Chai, R. In Proceedings of the American Head & Neck Society 2026.
Real-World Discordance between ctHPVDNA Liquid Biopsy and HPV Status in HPV-Positive OPSCC: A Prospective Single-Institution Study
Vang, H.; Bedi, N.; Chan, P.; Tsang, H.; Lee, J.; Chen, M.M.; Finegersh, A.; Soo, J.; Holsinger, F.C. In Proceedings of the American Head & Neck Society 2026.
Biomarkers for HPV+ OPSCC Recurrence and Survival: Early Results of U01 Funded Prospective Study
Yarbrough, W.; Schrank, T.; X, T.; Blumberg, J.; Hackman, T.; Lumley, C.; Patel, M.; Colaianni, A.; Ferris, R.; Van Abel, K.; et al. In Proceedings of the American Head & Neck Society 2026.
Patterns of Recurrence after Detection of Surveillance Circulating Tumor HPV DNA in P16+ Oropharyngeal Cancer
Lee, R.; Fortino, J.; Xu, M.; Wai, K.; Likhterov, I.; Heaton, C.; George, J.; El-Sayed, I.; Ha, P.; Algazi, A.; et al. In Proceedings of the American Head & Neck Society 2026.
Reduction in Circulating Tumor Tissue-Modified(TTMV)-HPV DNA Is Associated with Improved Long-Term Survival in Patients with Recurrent or Metastatic Head and Neck Squamous Cell Carcinoma Treated with Cetuximab and Nivolumab in a Phase II Clinical Trial
Sun, S.; Danan, D.; Kitrane, K.; Rocco, J.; Bonomi, M.; Saba, N.; Chung, C.; Park, R. In Proceedings of the American Head & Neck Society 2026.
Novel Baseline Quantitative MRI Relaxometry Parameters Predict Circulating Tumor DNA (ctDNA) Clearance on a Phase II HPV-Oropharynx Cancer (HPV-OPSCC) De-Escalation Study
Bakhtiar, M.; Guenette, J.P.; Madore, B.; Minken, J.; Mukundan, D.; Kaza, E.; Hanna, G.J.; Rettig, E.M.; Schoenfeld, J.D. International Journal of Radiation Oncology, Biology, Physics 2026, 125, e52–e53
Early Results of a Multisite Phase II Study of ctDNA-Guided Adjuvant Radiation for Patients with HPV-Related Oropharyngeal Cancer
Bates, J.E.; Stokes, W.A.; Remick, J.S.; Rudra, S.; McDonald, M.W.; Jethanandani, A.; Simon-Kamm, A.; Steininger, M.M.; Lockney, N.A.; Mannion, K.; et al. International Journal of Radiation Oncology, Biology, Physics 2026, 125, e30–e31
Comparative Performance of ctHPVDNA and Imaging Surveillance for Oropharyngeal Cancer Following Radiotherapy
Ding, Y.; Saba, N.F.; Jethanandani, A.; Boyce, B.J.; Bates, J.E.; Rudra, S.; Schmitt, N.C.; Patel, M.R.; Kaka, A.S.; Stokes, W.A. International Journal of Radiation Oncology, Biology, Physics 2026, 125, e75–e76
Circulating Tumor HPV DNA Driven Adjuvant Treatment Deintensification after Transoral Surgery for HPV-Positive Squamous Cell Carcinoma of the Oropharynx
Farlow, J.L.; Bhatt, N.M.; Chen, D.W.; Campbell, D.A.; Mantravadi, A.; Moore, M.; Durm, G.; Marin-Acevedo, J.; Langer, M.P.; Yesensky, J.; et al. International Journal of Radiation Oncology, Biology, Physics 2026, 125, e59–e60
Phase II Trial of Neoadjuvant Chemotherapy (NAC) Docetaxel-Cisplatin Alone or with Anti-Human Papillomavirus (HPV) Gene Therapy PRGN-2009 in Newly Diagnosed HPV-Associated Oropharyngeal Cancer (HPV-OPSCC) Patients
Floudas, C.S.; Valdez, J.; Wheatley, M.; Patel, R.; Thakkar, P.; Joshi, A.; Allen, C.T. International Journal of Radiation Oncology, Biology, Physics 2026, 125, e53
Prognostic Significance of Undetectable Baseline HPV Circulating Tumor DNA in Oropharyngeal Cancer
Fortino, J.A.; Sunder, A.; Lee, R.; Qian, A.; Algazi, A.P.; Kang, H.; Chow, M.; Xu, M.J.; Wai, K.C.; Ryan, W.; et al. J Clin Oncol 2026, 44, 240–240
Abstract 6842: ctDNA Precedes Imaging: A Predictive Model for Real-Time Treatment Adaptation in HPV-Associated Anal Squamous Cell Carcinoma
Havor, P.M.A.; Huffman, B.M.; Cleary, J.; Brady-Nicholls, R. Cancer Res 2026, 86, 6842
Extended Follow-up to Compare the PPV, NPV and Clinical Utility of Surveillance Circulating Tumor Tissue Modified Viral (TTMV)-HPV DNA in a Community-Based Head and Neck Cancer Program with Larger Academic Programs
Hockstein, L.A.; Shah, S.; Katzenberg, A.; Park, S.; Clements, L.; Desouza-Lawrence, L.; Amin, J.; Raben, A. International Journal of Radiation Oncology, Biology, Physics 2026, 125, e78
Real-World Evidence of Circulating Tumor Tissue–Modified Viral DNA (TTMV-HPV DNA) for Molecular Monitoring in HPV-Associated Cancers: Analysis of 100,000 Blood Tests
Jabalee, J.; Kuperwasser, C.; Naber, S.P.; Berger, B.; Gupta, P.B. International Journal of Radiation Oncology, Biology, Physics 2026, 125, e76–e77
Pilot Study of HB-200 in Patients with HPV16+ Head and Neck Squamous Cell Carcinoma and Detectable TTMV-HPVDNA after Definitive Therapy
Wong, W.; Ostrovnaya, I.; Riaz, N.; Sherman, E.J.; Dunn, L.; Lee, N.Y.; Wong, R.J.; Ho, A.L. J Clin Oncol 2026, 44, e18038–e18038
The Utility of Liquid Biopsy for Diagnosis and Surveillance of Non-Oropharyngeal HPV+ Squamous Cell Carcinoma of the Head and Neck
Cooke, P.; Chennareddy, S.; Kraft, D.; Correa, J.; Chen, S.; Kappauf, C.; Sindhu, K.; Ferrandino, R.; Misiukiewicz, K.; Bakst, R. In Proceedings of the COSM – AHNS 2025.
Determining the Relationships between Quantitative HPV DNA Liquid Biopsy Results and Circulating Immune Cell Populations in HPV-Positive Head and Neck Squamous Cell Carcinoma
Wofford, W.; Walker, A.; Albergotti, W.; Newman, J.; Kaczmar, J.; Chera, B. In Proceedings of the EUROGIN 2025.
Tumor Tissue Modified Viral (TTMV)-HPV DNA as a Biomarker of Treatment Efficacy for Definitive Chemoradiation in Anal Squamous Cell Carcinoma: A Step towards Truly Personalized Therapy
Agarwal, S.; Majeed, U.; Jones, J.C.; Stocchi, L.; May, B.; Koffler, D.; Merchea, A.; Colibaseanu, D.; DeLeon, M.; O’Donnell, C.; et al. J Clin Oncol 2025, 43, 10–10.
314 Ultrasound Guided Fine Needle Aspiration of Head and Neck Squamous Cell Carcinoma: Utilization of Rapid On-Site Evaluation (ROSE), Acquisition of Formalin Fixed Tissue and Liquid Biopsy for HPV Status Assessment
Benchbani, H.; Reynolds, J.; Patel, S.; Pirgousis, P.; Dahlen, H.; Colletti, S.; Asghar, I.; Nassar, A.; Agarwal, S. Laboratory Investigation 2025, 105.
Impact of Smoking Status on Circulating Tumor HPV DNA in a Diverse Population with Oropharyngeal Cancer
Ozair, M.Z.; Lambert, T.; Velten, C.; Brodin, P.; Nolasco, B.; Sehrawat, K.; Gjini, M.; Schiff, B.; Mehta, V.; Castellucci, E.; et al. International Journal of Radiation Oncology, Biology, Physics 2025, 123, e368–e369.
Integrating Radiomic Features and Circulating Tumor HPV DNA in Predictive AI Models for Personalized Treatment in HPV-Positive Oropharyngeal Cancer
Rentiya, Z.S.; Sousa, G.L.A. de; Debamita, K.; McLaughlin, C.; Wijesooriya, K. International Journal of Radiation Oncology, Biology, Physics 2025, 123, e374.
Initial Results of MC200710 Investigating Therapeutic Vaccine (PDS0101) Alone or with Pembrolizumab Prior to Surgery or Radiation Therapy for Locally Advanced HPV Associated Oropharyngeal Carcinoma, a Phase 2 Window of Opportunity Trial
Routman, D.M.; Price, K.A.R.; Asmus, E.; Foster, N.R.; Moore, E.J.; Price, D.L.; Tasche, K.; Yin, L.X.; McGarrah, P.W.; Fuentes Bayne, H.E.; et al. J Clin Oncol 2025, 43, 6061–6061.
1364P Circulating Tumor Tissue-Modified Viral HPV DNA Detection in Non-Oropharyngeal, HPV+ Head and Neck Cancers
Sheth, S.; Trivedi, S.; Perez-Melendez, Y.; Sargent, B.; Jabalee, J.; Kaczmar, J.; Dagan, R.; Chera, B.; Hanna, G.J. Annals of Oncology 2025, 36, S846–S847.
Efficacy of TTMV HPV DNA Testing for Surveillance in HPV + Head and Neck Cancer
Worona, L.A.; Sanchez, O.; Misiukiewicz, K.; Ramos, E.J.; Reed, S.; Bakst, R.L.; Sindhu, K.K.; Roof, S.; Westra, W.H.; Posner, M.R. J Clin Oncol 2025, 43, e18056–e18056.
Association of Circulating Tumor HPV DNA Score and Clinicopathologic Characteristics in HPV-Associated Oropharyngeal Cancer
Sharav, S.; Kerl, N.; Turner, J.; Jasper, S.; Houlton, J.; Neskey, D. In Proceedings of the AAO-HNSF 2024.
Beyond the Blade: Retropharyngeal Lymph Nodes and an Elusive Complete Resection
Crompton, D.J.; Holtzman, A.L. Int J Radiat Oncol Biol Phys 2024, 120, 10–11.
HPV-DNA Titers and Tumor Volume Correlation in Human Papillomavirus Related Oropharyngeal Squamous Cell Carcinoma
Devpura, S.; Ghanem, A.I.; Gilbert, M.; AlKhatib, S. a. R.; Bagher-Ebadian, H.; Tam, S.; Wu, V.; Thind, K.; Siddiqui, F. International Journal of Radiation Oncology, Biology, Physics 2024, 120, e747
Detectable Circulating Tumor Tissue Modified Viral (TTMV)-HPV DNA in Non-Oropharyngeal Head and Neck Cancers
Duffy, E.W.; Kaczmar, J.; Albergotti, W.; Kejner, A.; Newman, J.G.; Graboyes, E.M.; Tamblyn, A.; Chera, B.S. International Journal of Radiation Oncology, Biology, Physics 2024, 118, e28–e29.
Neoadjuvant Immunotherapy for Oropharynx Cancer: Correlative Studies and Long-Term Outcomes from the CIAO Trial
McGrail, D.; Li, K.; Nipper, A.; Johnson, F.M.; Santana, E.; Bell, D.; Lee, M.; Haymaker, C.L.; Garden, A.S.; Lin, S.-Y.; et al. J Clin Oncol 2024, 42, 6059–6059.
TTMV and Association with Relapse in Patients with HPV Related SCCHN Undergoing CRT
Misiukiewicz, K.; Ramos, E.J.; Worona, L.A.; Sindhu, K.K.; Bakst, R.L.; Roof, S.; Genden, E.M.; Westra, W.; Posner, M.R. J Clin Oncol 2024, 42, 6067–6067
Tracking Circulating Tumor HPV DNA with Early Cessation of Radiotherapy in Oropharynx Cancer
Rentiya, Z.S.; Garneau, J.; Kaur, V.; Read, P.W.; Kalman, N.S.; Fedder, K.L.; Dowling, E.; Shonka, D.C.; McLaughlin, C. International Journal of Radiation Oncology, Biology, Physics 2024, 120, e783–e784.
Abstract B022: Immediate Post-Treatment Detection of Minimal Residual Disease in HPV-Driven Oropharyngeal Cancer Is Associated with High Risk of Recurrence
Roof, S.A.; Jabalee, J.; Rettig, E.M.; Ferrandino, R.; Chen, S.; Posner, M.R.; Misiukiewicz, K.J.; Gender, E.M.; Chai, R.L.; Sims, J.; et al. Clin Cancer Res 2024, 30, B022.
The NAVigate-HPV Registry: A Comprehensive Biomarker Evidence Base for HPV-Driven Cancers
Roof, S.; Hanna, G.J.; Rettig, E.M.; Routman, D.M.; Holsinger, F.C.; Kalman, N.; Bhatia, A.K.; Patel, M.R.; Jabalee, J.; Yom, S.S.; et al. J Clin Oncol 2024, 42, e15045–e15045.
Clinical Utility of Pre-Treatment and Surveillance Circulating Tumor Tissue Modified Viral HPV DNA to Detect Recurrence in a Community Based HN Cancer Program
Russial, O.; Raben, A.; Shah, S.; Hockstein, N.; Park, S.; Clements, L.; Cormier, J. International Journal of Radiation Oncology, Biology, Physics 2024, 118, e75.
Clinical Utility of Circulating Tumor Tissue-Modified Viral HPV DNA Testing in HPV-Driven Oropharyngeal Cancer Arising in Women
Russial, O.; Raben, A.; Shah, S.; Kegelman, T. J Clin Oncol 2024, 42, e18032–e18032.
Abstract A007: Transient Elevation of ctHPVDNA in Human Papilloma Virus-Mediated Oropharynx Squamous Cell Carcinoma Patients Following Diagnostic Surgical Procedures
Vang, H.; Soo, J.; Bedi, N.; Garofalo, A.; Chen, M.M.; Finegersh, A.; Holsinger, F.C. Clin Cancer Res 2024, 30, A007.
The Preliminary Analysis of Circulating Human Papillomavirus DNA in a Phase III Trial of Concurrent Chemoradiation (CRT) with Intensity Modulated Proton Therapy (IMPT) vs. IMRT in Oropharyngeal Squamous Cell Carcinoma (OPSCC)
Wang, L.; Zhang, Y.; Ausat, N.; Hernandez, M.; Rosenthal, D.I.; Garden, A.S.; Ferrarotto, R.; Gunn, G.B.; Fuller, C.D.; Phan, J.; et al. International Journal of Radiation Oncology, Biology, Physics 2024, 120, S123–S124.
Detecting HPV-Driven Oropharyngeal Carcinoma Recurrence Using Circulating Tumor HPV DNA
Shakhtour, L.; Pershad, A.; Haroun, F.; Madani, M.; Shaver, T. In Proceedings of the AAO-HNSF 2023.
Association of Preoperative TTMV-HPV DNA Serologic Testing with Clinical Presentation and Demographics
Kappauf, C.; Ferrandino, R.; Chen, S.; Barlow, J.; Gold, B.; Berger, M.; Westra, W.; Teng, M.; Khan, M.; Posner, M.; et al. In Proceedings of the American Head & Neck Society 2023.
Molecular-Based Confirmation of the Neck Imaging Reporting and Data System (NI-RADS) in the Treatment of HPV+ Oropharynx Cancer
Stepp, W.; Morse, R.; Tasoulas, J.; Huang, B.; Oldan, J.; Shen, C.; Hackman, T. In Proceedings of the American Head & Neck Society 2023.
Proof of Concept: The Use of Circulating Viral DNA Blood Testing in the Management of HPV-Driven Sinonasal Malignancy
Jawad, B.A.; Farlow, J.; McCrary, H.; Vankoevering, K.; Carrau, R. In Proceedings of the Journal of Neurological Surgery Part B: Skull Base; Georg Thieme Verlag KG, February 2023; Vol. 84, p. P155.
679 Characterization of Tumor Specific CD8+ T Cell Responses in Patients with Recurrent/Metastatic HPV16-Positive Head and Neck Cancer Receiving HB-200 Monotherapy as Second or Later Line Treatment in a Phase 1 Study
Ho, A.L.; Pearson, A.T.; Fu, S.; Posner, M.R.; Adkins, D.; Nabell, L.; Niu, J.; Richardson, D.L.; Leidner, R.S.; Chung, K.; et al. J Immunother Cancer 2023, 11.
Definitive Chemoradiation Treatment Response Evaluation Using NI-RADS and ctHPVDNA for HPV-Associated Oropharyngeal Squamous Cell Carcinoma
Morse, R.; Stepp, W.H.; Oldan, J.; Huang, B.Y.; Tasoulas, J.; Chera, B.S.; Chen, X.; Hackman, T.; Shen, C. International Journal of Radiation Oncology, Biology, Physics 2023, 117, S150–S151.
921P HB-200 Arenavirus-Based Immunotherapy plus Pembrolizumab as a First-Line Treatment of Patients with Recurrent/Metastatic HPV16 Positive Head and Neck Cancer
Nabell, L.; Ho, A.L.; Posner, M.R.; Neupane, P.; Naqash, A.-R.; Wong, S.; Niu, J.J.; Pearson, A.T.; Laux, D.; Adkins, D.R.; et al. Annals of Oncology 2023, 34, S581–S582.
Association of Plasma Tumor Tissue Modified Viral HPV DNA (TTMV) with Tumor Burden, Treatment Type, and Outcome: A Translational Analysis from NRG-HN002
Yom, S.S.; Torres-Saavedra, P.A.; Kuperwasser, C.; Kumar, S.; Gupta, P.B.; Ha, P.; Geiger, J.L.; Banerjee, R.; Thorstad, W.; Blakaj, D.; et al. JCO 2022, 40, 6006–6006.
Evaluating a Clinically Validated Circulating Tumor HPV DNA Assay in Saliva as a Proximal Biomarker in HPV+ Oropharyngeal Squamous Cell Carcinoma
Gerndt, S.P.; Ramirez, R.J.; Wahle, B.M.; Kuperwasser, C.; Gunning, A.; Chaudhuri, A.A.; Zevallos, J.P. JCO 2021, 39, 6063–6063.
Pre-Treatment Circulating Tumor Human Papillomavirus (HPV) DNA and Recurrence Risk in HPV Associated Malignancy: Oropharyngeal, Anal Canal, and Cervical Cancer
Garda, A.E.; Jethwa, K.R.; Routman, D.M.; Kumar, S.; Chera, B.S.; Viehman, J.; Sandhyavenu, H.; Liu, M.C.; Laack, N.N.; Hallemeier, C.L.; et al. Int J Radiat Oncol Biol Phys 2020, 108, S6–S7.
